This excellent presentation from BIA Separations discusses how monoliths and chromatography can be used for fast, easy purification and production of high-yielding mRNA.
The isolation and linearization of the pDNA is covered first, followed by the four monolith column types used in the “toolbox” for the capture and polishing of the mRNA, namely:
- Oligo dT18
- C4 HLD
The chromatography chemistries discussed in the presentation are available in all the CIMmultus monolith columns size options, from 1 ml up to 800 ml column volumes.
For more information on the BIA Separations CIMmultus used in this presentation, go to our Columns & Media page.
You might be interested in these earlier posts relating to mRNA
Knauer has developed a technique for mass-producing lipid nanoparticles (LNPs) using impingement jet mixing technology (IJM). LPNs have been shown to be a suitable delivery
The purification of mRNA presents challenges due to its chemical structure and the contaminants present. These challenges, with important techniques and tips are covered in