The purification of mRNA presents challenges due to its chemical structure and the contaminants present. These challenges, with important techniques and tips are covered in this presentation by BIA-Separations. The main topics covered include how the chemical structure of mRNA presents purification challenges and how contaminants need to be constantly considered when developing a purification strategy.
The first section explores the advantages of laminar flow in monolith columns for the purification of large molecules such as mRNA.
The second section looks at the purification of mRNA; with its relative size, strong hydrogen bonding and likely contaminants all proving to be a challenge.
The last section looks at the chromatography purification options available, including hybridization-affinity, anion exchange, hydrogen bond, hydrophobic interaction and reverse phase.
The chromatography chemistries discussed in the presentation are available in all the CIMmultus monolith columns size options, from 1 ml up to 800 ml column volumes.
For more information on the sizes and types of CIMmultus columns available go to our Columns & Media page
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