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The purification of mRNA, and how to deal with impurities and contaminants

The purification of mRNA presents challenges due to its chemical structure and the contaminants present. These challenges, with important techniques and tips are covered in this presentation by BIA-Separations. The main topics covered include how the chemical structure of mRNA presents purification challenges and how contaminants need to be constantly considered when developing a purification strategy.

mRNA Toolbox Intro
mRNA Size Issues

The first section explores the advantages of laminar flow in monolith columns for the purification of large molecules such as mRNA.

mRNA chemistry challenge

The second section looks at the purification of mRNA; with its relative size, strong hydrogen bonding and likely contaminants all proving to be a challenge.

mRNA Hybridization Affinity Chromatography

The last section looks at the chromatography purification options available, including hybridization-affinity, anion exchange, hydrogen bond, hydrophobic interaction and reverse phase.

The chromatography chemistries discussed in the presentation are available in all the CIMmultus monolith columns size options, from 1 ml up to 800 ml column volumes.

For more information on the sizes and types of CIMmultus columns available go to our Columns & Media page

CIMmultus 1ml Monolith Column
CIMmultus 1ml Monolith Column
CIMmultus-4ml Monolith Column
CIMmultus-4ml Monolith Column
CIMmultus 8ml Monolith Column
CIMmultus-8ml Monolith Column

For a PDF copy (1.44 MB) of the presentation from BIA-Separations “Purification of single-stranded mRNA: The toolbox for the next generation”  CLICK HERE.

You might also be interested in this earlier post:

Posted in Chromatography