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How and When to use Peak Recycling to separate eluting peaks that overlap.

Peak Recycling Chromatogram

Peak recycling is a method where peaks that are eluting too close to each other in an isocratic separation are reloaded back onto the column. This can be done multiple times until baseline separation is achieved. It is a methodology that is well known in process chromatography.

The Infinite Column – Peak Recycling Elongates the Separation Length of the Column.

In this poster Knauer looks at automated peak recycling using the isocratic separation of the two (+/-)-α-Tocopherol isomers as a practical example. The method only requires a T-connector and a multi-position motorized valve.

Fractionation and Recycling Modes

CLICK HERE for pdf copy of the poster (1,773 KB)

Posted in HPLC Systems